Spore syringes can be used to inoculate a substrate directly or via agar plates for a more controlled approach.
Basic steps:
- Work in the cleanest environment possible. A still air box is recommended.
- Flame-sterilise the needle before use.
- Inject 2-3 ml of spore solution into your substrate or agar plate.
- Store at 24-28 degrees C and wait for colonisation.
Sterility is everything. One shortcut here and contamination takes over.